Table 2. FM1-43FX uptake in HC-like cells.
| ES-M | ST2-CM | |
|---|---|---|
| FM1-43FX+ (%)a | 0.52±0.1 | 21.3±1.6 |
| Brn3c+ (%)b | 0.45±0.1 | 19.2±2.1 |
| FM1-43FX+/Brn3c+ (%)c | 17.2±0.2 | 92.3±4.5 |
Abbreviation: ST2-CM, conditioned medium from ST2 stromal cells
a
The percentages were quantified using FM1-43FX-positive cells and nuclei stained by DAPI (n=15)
b
The percentages were quantified using Brn3c-immuno-positive cells and nuclei stained by DAPI (n=15)
c
Brn3c immunostaining was performed after FM1-43FX dye treatment and fixation. The percentages of FM1-43FX-positive cells in Brn3c-immuno-positive cells are shown (n=15)