Skip to main content
. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2010 Dec 15;94(0):44–52. doi: 10.1016/j.prostaglandins.2010.12.003

Figure 3.

Figure 3

Suppression of Sptlc2 expression by Sptlc2 siRNA and altered sphingolipid profile. Raw264.7 cells were transfected with Sptlc2-siRNA for 72 hrs and Sptlc2 proteins were immunoblotted with Sptlc2-antibody (A). Cells transfected with the control siRNA (siControl) and Sptlc2-siRNA (siSptlc2) were incubated with LPS for 8 hrs. Sphingolipids were extracted and analyzed by LC/MS/MS as described in Materials and Methods. Total ceramides (B) and SM (C) were quantified. n=3, mean ± SEM, *p<0.05 vs. control. § p<0.05 vs. LPS. Results are representative of three independent experiments.