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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2010 Dec 15;94(0):44–52. doi: 10.1016/j.prostaglandins.2010.12.003

Figure 6.

Figure 6

Promoter analysis of Sptlc2 by NFκB in Raw264.7 cells. Sptlc2 reporter construct with various lengths of Sptlc2 promoter were co-transfected with pcDNA2.1 empty vector or pcDNA2.1-p65 vector and the luciferase activity was measured (A). Raw264.7 cells were incubated with 1 μg/ml LPS for 8 hrs and chromatin was immunoprecipitated by anti-p65 antibody. Immunoprecipitaed chromatin DNA was fragmented by sonication to produce 0.2~0.4 kb DNA fragments. PCR was performed to measure the promoter region of -449~-250 and -845~-650 (B). n=3, mean ± SEM. §p<0.05 vs. empty control. #p<0.05 vs. -849 promoter. *p<0.05 vs. -449 promoter. Results are respresentative of two independent experiments.