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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Arthritis Rheum. 2012 Jul;64(7):2289–2299. doi: 10.1002/art.34411

Figure 2. WISP3/CCN6 and metalloproteinase expression in clonal cell lines.

Figure 2

WISP3/CCN6 and metalloproteinase expression were determined in clonal cell lines stably over-expressing the empty vector pcDNA3.1 hygro (vector) or pcDNA3.1 hygro + WISP3/CCN6 (WISP3). (A) Top: WISP3/CCN6 steady state mRNA expression measured by real time RT-PCR. Bottom: anti-WISP3/CCN6 western blot of cell lysates. (B–C) Real time RT-PCR for metalloproteinase mRNA. (B) ADAMTS5 (top) and ADAMTS4 (bottom) expression. (C) MMP10 (top) and MMP1 (bottom) expression. *, **, *** = a significant difference where P is ≤ 0.05, 0.01 and 0.001 respectively. All real time RT-PCR was normalised to expression of the house keeping gene 18S. Each bar is the mean of the fold change in three clones ± s.e.m compared to the clones stably over-expressing the empty vector.