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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Arthritis Rheum. 2012 Jul;64(7):2289–2299. doi: 10.1002/art.34411

Figure 3. ADAMTS5 and MMP10 expression in monolayer versus 3D culture in the presence and absence of cytokines.

Figure 3

Real time RT-PCR normalised to 18S. (A) ADAMTS5 and MMP10 expression in clonal cell lines stably over-expressing the empty vector (vector) or in clonal cell lines stably over-expressing WISP3/CCN6 (WISP3) grown in monolayer or in suspension over polyhema. Each bar is the mean of the fold change in three clones ± s.e.m compared to the clones stably over-expressing the empty vector in monolayer culture. (B) ADAMTS5 and MMP10 expression in one empty vector clone (vector) and one WISP3/CCN6 clone (WISP3) grown in monolayer in serum free medium (SF) or SF supplemented with 5ng/ml IL-1α and 10ng/ml OSM (IL-1+OSM). (C) ADAMTS5 and MMP10 in one empty vector clone (vector) and one WISP3/CCN6 clone (WISP3) grown in suspension over polyhema in serum free medium (SF) or SF supplemented with 5ng/ml IL-1α and 10ng/ml OSM (IL-1+OSM). Each bar in (B) and (C) is the mean of the fold change in three wells ± s.e.m compared to cells stably over-expressing the empty vector in control conditions (SF, monolayer culture). *, **, *** = significant difference in expression where P is ≤ 0.05, 0.01 and 0.001 respectively.