Table 1. Changes in steady state mRNA levels of metalloproteinases in WISP3/CCN6 over-expressing clonal cell lines versus control clones.

The expression of MMP and ADAMTS mRNA in three empty vector C-28/I2 clones and three WISP3/CCN6 over-expressing C-28/I2 clones was measured by real time RT-PCR and normalised to 18S expression. Fold changes in gene expression in the WISP3/CCN6 clones compared to the empty vector clones are indicated where differences in expression were significant (p ≤ 0.05).

Metalloprotienase	Regulation in WISP3/CCN6 over-expressing cells
MMP1	↑ 13 Fold	* P<0.05
MMP2	↓ 2 Fold	* P<0.05
MMP3	No effect
MMP7	No effect
MMP8	No expression
MMP9	No effect
MMP10	↑ 14 Fold	** P<0.01
MMP11	↓ 1.5 Fold	* P<0.05
MMP12	No expression
MMP13	No effect
MMP14	↓2 Fold	* P<0.05
MMP15	↓ 2.5 Fold	** P<0.01
ADAMTS1	No effect
ADAMTS4	↓ 4 Fold	* P<0.05
ADAMTS5	↓ 9 Fold	*** P<0.001
ADAMTS8	No expression
ADAMTS9	No effect
ADAMTS15	No effect