Table I. Peptide analysis of HIV-1 Vif.

Peptides fragments were identified by mass spectrometry (MALDI-TOF-MS, LC-ion trap-MS, or LC-QTof-MS [Q-Tof] indicated by MS) from uncross-linked (UnX), cross-linked monomer (Mono), dimer, or trimer and digested with either trypsin (T) or chymotrypsin (C). Italics indicate identification by Q-Tof; bold indicates identification by both Q-Tof and LC-ion trap-MS.

UnX-T	UnX-T- MS	UnX-C	Mono- T	Mono-T- MS	Mono-C	Dimer-T	Dimer-T- MS	Dimer- C	Trimer-T	Trimer-T- MS	Trimer- C
1–15			1–15			1–15			1–15
				5–19			5–19			5–19
		12–21			12–21
16–22						16–22			16–22
23–26			23–26			23–26			23–26
					22–30			22–30			22–30
	23–34
23–36			23–36			23–36			23–36
27–41
		30–39
		31–39			31–39
					31–40			31–40
35–41			35–41			35–41			35–41
37–41	37–41		37–41	37–41		37–41	37–41		37–41	37–41
		39–44			39–44			39–44			39–44
42–50	42–50		42–50	42–50		42–50	42–50		42–50
42–63			42–63			42–63			42–63
51–63	51–63		51–63	51–63		51–63	51–63		51–63	51–63
64–77	64–77		64–77	64–77		64–77	64–77		64–77	64–77
		70–79			70–79			70–79			70–79
		70–89
		71–79			71–79						71–79
78–90	78–90		78–90	78–90		78–90	78–90		78–90	78–90
78–91	78–91		78–91						78–91
	78–92
	93–122
	94–121			94–121			94–121
	94–122			94–122			94–122
								95–106			95–106
					112– 115
	122–132			122–132						122–132
123– 132	123–132		123– 132	123–132		123– 132	123–132		123–132	123–132
								126– 135			126–135
	133–157
		136–147			136– 147			136– 147			136–147
	142–157			142–157			142–157
		148–174
		151–174			151– 174
	158–168			158–168			158–168			158–168
	159–168			159–168			159–168			159–168
	159–173
	161–168									161–168
	161–173
174– 184	174–184		174– 184	174–184		174– 184			174–184
					175– 192			175– 192
	182–192
185– 192			185– 192			185– 192			185–192