Table II.

Cross-links of HIV-1 Vif. Cross-linked residues identified by MALDI-TOF-MS and heavy water labeling of trypsin-digested monomeric, dimeric or trimeric forms of HIV-1 Vif. The cross-linking agent EDC specifically links lysine (K) to either aspartic acid (D) or glutamic acid (E). If more than one lysine or acid is within an identified fragment, all are listed. Linking residues are listed sequentially. Cross-linked residues in bold appear in Figure 4. Italics indicate that experimental molecular weights were consistent with theoretical molecular weights of both cross-links.

Monomer		Dimer		Trimer
K26, K34	E171, D172
Termini	E45	Termini	E45	Termini	E45
Termini	D61, E76	Termini	D61, E76	Termini	D61, E76
E2	K26, K34	E2	K26, K34	E2	K26, K34
E54, D61	K176, K179	E54, D61	K176, K179	E54, D61	K176, K179
E2	K141	E2	K141	E2	K141
E2	K176, K179, K181	E2	K176, K179, K181	E2	K176, K179, K181
D14	K26, K34	D14	K26, K34	D14	K26, K34
K22, K26	E45	K22, K26	E45	K22, K26	E45
K26, K34	D78, E88	K26, K34	D78, E88	K26, K34	D78, E88
E45	K91, K92	E45	K91, K92	E45	K91, K92
E45	K92	E45	K92	E45	K92
D78, E88	K91, K92	D78, E88	K91, K92	D78, E88	K91, K92
E171, D172	K181	E171, D172	K181	E171, D172	K181
		K34	E134	K34	E134
		E45	K176, K179	E45	K176, K179
		D61	K160, K168	D61	K160, K168
		K92	E171, D172	K92	E171, D172
				D14	K158, K160, K168
				K34	Termini
				K158, K160, K168	E171, D172
				K158, K160, K168	Termini
				K160, K168, E171, D172	Termini
				K160, K168, E171, D172, K176, K179	Termini
				E171, D172	K181