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Immunofluorescent staining of cultured mononculear cells for endothelial antigens CD31 and endothelial nitric oxide synthase (eNOS). CD31 and eNOS staining was observed in a subset of cells. A representative sample from a subject with Hemoglobin SS is shown. (a) shows phase contrast image and (c) shows CD31 staining (green) with nuclei stained with DAPI (blue) in the same field. (b) Shows phase contrast and (d) shows eNOS staining (green) for the same sample.
