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ELISA was used to measure ET-1 peptide levels in the inner medulla (Panel A, n=4) and cortex (Panel B, n=6) of Per1 KO and WT mice at midnight or noon. Data were analyzed using 2-way ANOVA (SigmaPlot), with significant genotype (*P<0.05) and time (†P<0.05) effects. There was not a statistically significant interaction between genotype and time.
