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. 2012 Feb 8;44(5):319–329. doi: 10.3858/emm.2012.44.5.036

Figure 2.

Figure 2

Characteristics of FITC-VDBP uptake. RLE-6TN cells were incubated in serum-free medium at 37℃ for 2 h with FITC-VDBP over a concentration range of 100-600 nM (A), and for a range of times from 30 to 150 min with 400 nM FITC-VDBP (B). Serum-starved RLE-6TN cells were preincubated with a range of doses of 0.5-4 µM native VDBP for 30 min and then incubated with 400 nM FITC-VDBP at 37℃ for 2 h (C). RLE-6TN cells were incubated in serum-free medium at 37℃ for 2 h with 400 nM FITC-VDBP. The cells were then washed with PBS and treated with 1.2 mg/ml trypan blue. Then, FITC intensity was quantified by immunofluorescence assay as described in the Materials and Methods section. Fluorescence intensities of the cells were counted using an image browser, and data are presented as the percent intensity (D). All data are representative of three independent experiments. Values represent the means ± SEM.