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. 2012 Feb 9;44(5):340–349. doi: 10.3858/emm.2012.44.5.038

Figure 2.

Figure 2

The expression of the pro-inflammatory cytokines (IL-12 and TNF-α) and the anti-inflammatory cytokine IL-10 was enhanced by FAP treatment in a TLR4-dependent manner, and GSK-3 is involved in the effect of FAP. (A) TLR4+/+ and TLR-/- BMDCs were stimulated with FAP (500 ng/ml). After 24 h, the concentrations of IL-12, TNF-α, and IL-10 were measured in the culture supernatants by ELISA. The mean (SEM) values shown represent 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with untreated TLR4+/+ BMDCs. (B) BMDCs were pretreated with or without the indicated GSK-3 inhibitor (SB415286) concentration for 30 min and then harvested after 24 h of incubation with FAP (500 ng/ml). The cytokine concentrations in culture supernatants were measured by ELISA. The mean ± SEM values shown represent 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with untreated BMDCs.