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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Circ Res. 2012 Apr 3;110(10):1294–1302. doi: 10.1161/CIRCRESAHA.112.267310

Figure 5.

Figure 5

Neutrophil adhesion and survival in KLF2-deficient mice. (A) Whole blood neutrophils pooled from male and female mice were analyzed for cell surface expression of integrin receptor CDllb in fasted and hypertriglyceridemic state; n = 10 per group. Cells were gated to identify the neutrophil population (Ly6Gpos:Ly6Cpos) and analyzed for surface expression of CD11b during fasting conditions or two hours after an oral lipid gavage. Neutrophil expression of CD11b is shown in representative histogram plots with the mean ±SEM of the median fluorescence intensity (MFI) displayed (inset). (B) In vitro adhesion of peritoneal neutrophils to TNFα-activated bEND.3 endothelial cells. (C) Viability of peritoneal neutrophils cultured in IMBM containing 10% serum (percent annexin V and propidium iodide negative cells). *P < 0.05, **P < 0.01.