Table 1.

Relative luciferase activities and Tms, ΔGs, and Kds at seed regions of class I and II siRNAs.

	Luciferase activity (% at 50 nM)	Seed region GC number	Tm 2–8 (°C)	ΔG 2–8 (kcal/mol)	Kd (M)
CLASS I
siLuc-309	76	0	−8.1	−7.2	5.3 × 10−6
siVIM-812	95	1	8.8	−9.3	1.5 × 10−7
siGRK4-934	102	1	6.7	−9.6	9.2 × 10−8
siOct-821	64	2	12.2	−10.3	2.8 × 10−8
siLuc-774	75	2	14.6	−10	4.7 × 10−8
siVIM-1128	79	3	21.2	−12.8	4.1 × 10−10
siLuc2-153	52	3	21.0	−11.7	2.6 × 10−9
siVIM-596	49	3	26.4	−11.6	3.1 × 10−9
siOct-797	29	3	25.7	−12.4	8.1 × 10−10
siVIM-270	25	3	26.2	−12.2	1.1 × 10−9
siLuc-36	49	4	28.4	−13	3.0 × 10−10
CLASS II
siGRK4-189	9	4	40.1	−15.5	4.3 × 10−12
siLuc-1120	44	5	42.3	−16.7	5.7 × 10−13
siLuc-49	6	6	46.3	−17.6	1.3 × 10−13
siLuc-1048	30	6	49.7	−17.8	8.9 × 10−14
siLuc-1063	18	7	54.5	−18.6	2.3 × 10−14
siLuc-1430	20	7	54.5	−18.6	2.3 × 10−14