Table 3.

Relative luciferase activities and Tms, ΔGs, and Kds at seed regions of class I and II chiRNAs.

	Luciferase activity (% at 50 nM)	Seed region GC number	Tm 2–8 (°C)	ΔG 2–8 (kcal/mol)	Kd (M)
CLASS I
chiLuc-309	103	0	−12.2	−4.9	2.6 × 10−4
chiVIM-812	94	1	−0.5	−5.5	9.3 × 10−5
chiGRK4-934	95	1	0.2	−5.6	7.3 × 10−5
chiOct-821	95	2	4.7	−7.1	6.2 × 10−6
chiLuc-774	65	2	−4.8	−6.8	1.0 × 10−5
chiVIM-1128	79	3	8.8	−7.9	1.6 × 10−6
chiLuc2-153	91	3	5.4	−7.8	1.9 × 10−6
chiVIM-596	63	3	8.8	−7.8	1.9 × 10−6
chiOct-797	84	3	1.3	−7.8	1.9 × 10−6
chiVIM-270	78	3	3.1	−7.6	2.7 × 10−6
chiLuc-36	68	4	19	−8.0	1.4 × 10−6
CLASS II
chiGRK4-189	12	4	28.1	−9.6	9.2 × 10−8
chiLuc-1120	69	5	31.1	−9.8	6.5 × 10−8
chiLuc-49	31	6	30.4	−11.0	8.6 × 10−9
chiLuc-1048	16	6	45.2	−9.7	7.7 × 10−8
chiLuc-1063	58	7	41.4	−12.0	1.6 × 10−9
chiLuc-1430	53	7	35	−12.0	1.6 × 10−9