Figure 1. Overexpression of ChREBP in livers of mice leads to the induction of the entire lipogenic and esterification program.

C57BL/6J mice were injected intravenously with a single dose of 5 × 10⁹ pfu of GFP or ChREBP adenovirus at day 1. Four weeks later, mice were sacrificed and analyses were performed. (A) Ser196 phosphorylation level of ChREBP in livers of overnight fasted GFP and ChREBP mice. A representative Western blot is shown (n = 10–12 group). (B) Quantification of the ratio of Ser196 ChREBP phosphorylation compared with total ChREBP protein content is shown. ***P < 0.001 ChREBP versus GFP mice. (C) Nuclear ChREBP and SREBP-1c protein content in nuclear extracts from fed GFP versus ChREBP mice. Lamin A/C antibody was used as a loading control. A representative Western blot is shown (n = 10–12/group). mSREBP-1c, mature SREBP-1c. (D) Total ACACA, FASN, and SCD1 protein content in liver lysates from fed GFP and ChREBP mice. β-Actin antibody was used as a loading control. A representative Western blot is shown (n = 10–12/group). (E) qRT-PCR analysis of ChREBP, SREBP-1c, LXR, Pparg, Gck, Pklr, Acaca, Fasn, Scd1, Elovl6, and GPAT in livers of GFP versus ChREBP mice. Results are the mean ± SEM (n = 10–12/group). *P < 0.05, **P < 0.01 ChREBP versus GFP mice.