Figure 1.

Fluorescence-visualized synaptic proteins. A, A schema of constructs to express CFP, YFP, and mCherry (left). Established stable lines were crossed so that a single larva expresses the three transgenes (right). The displayed larva (3 dpf) expressed rapsyn-CFP, AChRδ-YFP, and mCherry, and the fluorescence was visualized with corresponding filter cubes. Scale bar, 500 μm. B, Using confocal microscopy, three signals overlapped in 3 dpf larvae. C, The larva shown in the top panels lacked AChRδ-YFP, and the larva in lower panels lacked rapsyn-CFP. Signals corresponding to the missing transgenes were blank, showing the lack of signal bleed-through. Scale bars: B, C, 50 μm.