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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: J Biol Chem. 2007 Jun 1;282(32):23129–23139. doi: 10.1074/jbc.M701857200

FIGURE 2. AAEK1 and −2 inhibit different sortases from bacilli and staphylococci.

FIGURE 2

S. aureus SrtA (A) and B. anthracis SrtA (B), SrtB (C), or SrtC (D) were incubated with increasing concentrations of AAEK1 (●) or AAEK2 (○) followed by the addition of substrate (a-LPETG-d for SrtA, a-KTDNPKTGDEA-d for SrtB, or a-GEKLPNTASNN-d for SrtC) and fluorescence measurements. IC50 is the concentration of compound that inhibits 50% of sortase activity and is displayed in micromolar in the upper right corner. Data were fit using an nonlinear least squares method in GraphPad Prism®, and represent the mean of three independent determinations.