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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: J Biol Chem. 2007 Jun 1;282(32):23129–23139. doi: 10.1074/jbc.M701857200

FIGURE 3. AAEK1generates a covalent adduct with Cys-184 of S. aureus SrtA.

FIGURE 3

S. aureus wild-type or mutant SrtA (C184A) were incubated with (B, C, and E) or without (A and D) AAEK1, followed by digestion with trypsin. Reaction products were separated by reversed-phase high-performance liquid chromatography and collected fractions subjected to MALDI-TOF MS. Compounds with m/z 1693.78 (fraction 54) and m/z 1555.74 (fraction 50) correspond to tryptic peptides encompassing the modified and unmodified active site Cys-184 of sortase, respectively.