Table 2.
In situ proteolysis of proteins that had previously formed poor crystals
| Expasy ID | Fragment | GI | Protease | Crystals | Diffraction | Structure progress |
|---|---|---|---|---|---|---|
| NE2398 | 1–146 | gi|30250323 | C | C | 1.8 Å, C | Solved: PDB code 2RC3 |
| Atu0899 | 1–311 | gi|17934807 | C | C | 2.0 Å, C | Solved: PDB code 2R8W |
| Atu2452 | 1–247 | gi|17936334 | C | C | 2.9 Å, C | Solved: PDB code 2R8B |
| Atu0870 | 1–256 | gi|17934778 | C | C | 2.1 Å, C | Solved: PDB code 2P35 |
| HP0029 | 1–218 | gi|15644662 | C | C | 2.0 Å, C | Solved: PDB code 2QMO |
| Atu0299 | 1–198 | gi|17934215 | C | C | 2.1 Å, C | Solved: PDB code 2QNI |
| Atu0434 | 1–370 | gi|17934348 | C, T | C, T | 2.7 Å, T | Solved: PDB code 2R9Q |
| Atu0238 | 1–297 | gi|17934154 | C, T | C, T | 2.0 Å, C | Data collected, crystal twinned |
| Atu0418 | 1–293 | gi|17934332 | C, T | C, T | 2.9 Å, C | Data collected, multiple crystals |
| Atu1003 | 1–176 | gi|17934911 | C, T | C, T | 2.9 Å, C | Data collected, multiple crystals |
| Atu0443 | 1–231 | gi|17934356 | C, T | C | 3.0 Å, C | Data collected, weak anomalous signal |
| Atu1358 | 1–169 | gi|17935258 | C | C | 2.4 Å, C | Waiting for data collection |
| Atu0854 | 1–164 | gi|17934762 | C | C | 2.4 Å, C | Waiting for data collection |
| Atu2438 | 1–215 | gi|17936320 | C, T | C, T | 2.5 Å, C | Waiting for data collection |
| SCO1463 | 1–360 | gi|21219965 | C | C | 7.8 Å, C | |
| rha05074 | 95–247 | gi|111018552 | C | C | NA | |
| Atu2132 | 1–335 | gi|17936016 | C | C | NA | |
| Tbd_0951 | 98–275 | gi|74316969 | C, T | C, T | NA | |
| Atu2821 | 1–230 | gi|17936696 | C, T | C | NA | |
| SCO0155 | 1–207 | gi|21218712 | C, T | C | NA | |
| SCO5636 | 1–259 | gi|21223987 | C, T | C | NA | |
| Atu2572 | 1–393 | gi|17936447 | C, T | C | NA | |
| SCO7694 | 1–190 | gi|21225954 | C, T | C | NA | |
| Atu1730 | 1–281 | gi|17935623 | C | C | NA | |
| SAV6650 | 1–334 | gi|29833192 | C, T | C | NA | |
| SCO3259 | 1–259 | gi|21221692 | C | C | NA | |
| rha02449 | 2–142 | gi|111017506 | C, T | C, T | NA | |
| Atu2110 | 1–413 | gi|17935994 | C, T | No | ||
| SCO0775 | 1–149 | gi|21219298 | C | No | ||
| SCO7645 | 1–237 | gi|21225906 | C, T | No | ||
| Atu2345 | 1–348 | gi|17936228 | C, T | No | ||
| SCO5231 | 1–254 | gi|21223599 | C | No | ||
| Atu1214 | 1–229 | gi|17935119 | C, T | C | NA | |
| SCO3315 | 1–230 | gi|21221746 | C, T | C | NA | |
| O31761 | 76–241 | gi|2634053 | C, T | T | NA |
Structures are shown in Supplementary Figure 1c–i. Proteases used: chymotrypsin (C) or trypsin (T). NA; the crystals were too small to be tested by X-ray diffraction.