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. 2012 Jun 1;86(6):943–954. doi: 10.4269/ajtmh.2012.11-0797

Figure 1.

Figure 1.

PFE0565w transcripts are present in the P. falciparum erythrocytic stages, including gametocytes. Primers specific for PFE0565w were used to amplify cDNA products (RT+), indicating the expression of the gene in both mixed erythrocytic stages (ES) and gametocyte stages (Gam) of infection. A 1,146 base pair product was the expected size and was amplified in mixed ES and Gam stages; however there also was an unpredicted product amplified in both the mixed ES and Gam. This product was sequenced and was confirmed to be PFE0565w, missing base pairs 291–988 (the resulting sequence does not translate into a protein). Genomic DNA (gDNA) was used as a positive control and a no reverse-transcriptase (RT–) reaction was used as a negative control to show that the RNA was not contaminated with gDNA. PFE0565w does not contain an intron and the arrow indicates the predicted PFE0565w RT-PCR product and the asterisks indicate the alternative PFE0565w RT-PCR products. This figure is a representative image of three biological replicates of both mixed erythrocytic stages and gametocytes.