Figure 3.

PFE0565w transcript is present in both the P. falciparum oocyst sporozoite (A) and salivary gland sporozoite (B) stages, as shown by RT-PCR. Primers specific for PFE0565w were used to amplify cDNA fragments of the correct product size (RT+), indicating the expression of the gene during the sporozoite stages of infection. Genomic DNA (g+) was used as a positive control and a no reverse-transcriptase (RT–) reaction was used as a negative control to show that the RNA was not contaminated with genomic DNA. PFE0565w does not contain an intron and the arrows indicate the PFE0565w RT-PCR products. This figure is a representative image from two biological replicates using oocyst sporozoites and three biological replicates using salivary gland sporozoites.