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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: Cell. 2007 Sep 21;130(6):1095–1107. doi: 10.1016/j.cell.2007.07.035

Figure 1. Nampt Is a Stress- and Nutrient-Responsive Gene that Protects Cells against the Genotoxic Agent MMS.

Figure 1

(A and B) Nampt levels in human fibrosarcoma HT1080 cells in the presence or absence of 10% FBS (A) or of liver tissue extracts from fed or 2 day-fasted Sprague-Dawley rats (B). Actin and tubulin were used as loading controls.

(C and D) Nampt protein (C) and mRNA levels of Nampt (D) in livers of fasted rats (n = 4; bars represent the mean of three experiments ± standard deviations [SD] using Student’s t test). (E) Western blot of Nampt in primary rat cardiomyocytes under hypoxia and/or serum starvation.

(F and G) Survival of HT1080 cells stably expressing human (F) or transiently expressing mouse Nampt (G) following treatment with 1.2 mM methylmethanesulfonate (MMS).

(H) Survival of human kidney HEK293 cells stably expressing human Nampt treated with MMS as in (F).

Always, bars represent the mean of three experiments ± SD.