Skip to main content
. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: Cell. 2007 Sep 21;130(6):1095–1107. doi: 10.1016/j.cell.2007.07.035

Figure 2. Nampt Protects against Apoptotic Cell Death Induced by Topoisomerase Inhibitors.

Figure 2

(A) Sensitivity of HT1080 with siRNA-mediated knockdown of NAMPT after MMS exposure.

(B) Stable overexpression of Nampt enhances survival of HEK293 cells following MMS treatment and the effect is blocked by the Nampt-inhibitor FK866.

(C) Survival of WT or Nampt knockdown HT1080 cells after serum deprivation for 22 hr and then exposure to MMS for 17 hr. Serum deprivation upregulates Nampt and enhances survival of WT but not Nampt knockdown HT1080 cells.

(D and E) Survival of HEK293 stably overexpressing Nampt (D) or HT1080 with siRNA knockdown of Nampt (E) following etoposide treatment.

(F) Survival of HT1080 Nampt knockdown cells after camptothecin treatment. Apoptosis was assessed by western blot analysis of cleaved Caspase-3.

Bars represent the mean of three experiments ± SD.