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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: Biochim Biophys Acta. 2011 Oct 17;1822(2):185–195. doi: 10.1016/j.bbadis.2011.10.008

Fig. 2.

Fig. 2

Berberine Protects from obesity-induced skeletal muscle mitochondrial dysfunction in Sprague–Dawley Rats. (A) State 3 respiration and FCCP-induced respiration of isolated skeletal muscle mitochondria from rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) measured polarographically and expressed in nAtomsO/min/mg protein (n=5 experiments **p<0.01 versus Ctl, # p<0.05 versus Ctl, §§ p<0.01 versus HFD, + p<0.05 versus HFD). (B and C) Succinate Dehydrogenase (B) and Cytochrome c Oxidase (C) activity in skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) measured polarographically and expressed in nAtomsO/min/mg protein (n=4 experiments *p<0.05 versus Ctl, ## p<0.01 versus HFD). (D) ATPase activity measured spectrophotometrically in skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) expressed in nmolPi/mgprotein/min (n=4 experiments *p<0.05 versus Ctl, # p<0.05 versus HFD). (E) ATP content in isolated mitochondria from skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) (n=5 experiments p≥0.05). (F) Amount of oxidative fibers analyzed by the ratio of Troponin 1 slow and Troponin 1 fast mRNA measured by means of quantitative RT-PCR in skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+ BRR) (n=4 experiments p≥0.05). (G) MyHCIIa and MyHCIIb mRNA analyzed by means of quantitative RT-PCR in skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) (n=4 experiments ** p<0.01 versus Ctl, # p<0.05 versus HFD). (H) Citrate Synthase activity measured spectrophotometrically in skeletal muscle of rats fed with Chow diet (Ctl), High Fat Diet (HFD) and High Fat Diet supplemented with BBR (HFD+BRR) expressed in nmol.mim.ml (n=4 experiments *p<0.05 versus Ctl, # p<0.05 versus HFD). All data represent mean±SEM.