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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: Biochim Biophys Acta. 2011 Oct 17;1822(2):185–195. doi: 10.1016/j.bbadis.2011.10.008

Fig. 6.

Fig. 6

Berberine increases NAD+/NADH ratio through induction of NAMPT expression in C2C12 cells. (A) NAD+/NADH ratio measured spectophotometricaly in C2C12 myotubes treated for 96 h with hyperglycemia and exposed to 5 μM BBR (n=4 experiments ***p<0.001 Ctl, ## p<0.01 versus 25 mM). (B) NAD+/NADH ratio measured spectophotometricaly in C2C12 myoblasts treated for 6 h, 12 h and 24 h with 5 μM BBR (n=4 experiments *p<0.05 **p<0.01 versus Ctl). (C) NAMPT mRNA analyzed by means of quantitative RT-PCR in C2C12 cells after 6 h, 12 h and 24 h of treatment with 5 μM BBR. Relative expression values of the untreated cells were taken as 1.0. (n=4 experiments *p<0.05 **p<0.01 versus Ctl). (D) PGC-1alpha, PGC1-beta, NRF-1, NRF-2, TFAM, NDUFS8, ND1, COX5b, COX1 mRNA analyzed by means of quantitative RT-PCR in C2C12 cells after 12 h of treatment with 5 μM BBR or 5 μM BBR+10 nM FK866. Relative expression values of the untreated cells were taken as 1.0. (n=4 experiments *p<0.05 **p<0.01 ***p<0.001 versus Ctl, # p<0.05 ## p<0.01 ### p<0.001 versus BBR). All data represent mean±SEM.