(A) Only PC-3/Mc cells, but not PC-3/S cells, colonized lungs
after joint i.v. injection. PC-3/S, but not PC-3/Mc, cells also expressed
Renilla luciferase. Firefly luciferase, but not
Renilla, signal was detected in lung tumors. In
parallel, GFP (expressed by GFP-PC-3/Mc cells) or RFP (expressed by
RFP-PC-3/S cells) was visualized microscopically. Only GFP signal, but not
RFP signal, was detected in lung tumors. (B) Only PC-3/Mc
cells, but not PC-3/S cells, colonize adrenal glands after i.c. joint
inoculation. Thirty-three days after inoculation, mice were sacrificed and
adrenal metastatic tumors frozen and processed for fluorescent visualization
of GFP or RFP and for immunofluorescent detection of firefly luciferase (as
a marker common to both cell types). Samples were counterstained for nuclei
with DAPI. Only GFP signal, but not RFP signal, was detected in adrenal
metastases. Scale bars: 50 μm.