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. 2012 Apr 16;122(5):1849–1868. doi: 10.1172/JCI59218

Figure 12. Metastases formed after joint injection of PC-3/Mc and PC-3/S cells contain exclusively epithelial PC-3/Mc cells.

Figure 12

(A) Only PC-3/Mc cells, but not PC-3/S cells, colonized lungs after joint i.v. injection. PC-3/S, but not PC-3/Mc, cells also expressed Renilla luciferase. Firefly luciferase, but not Renilla, signal was detected in lung tumors. In parallel, GFP (expressed by GFP-PC-3/Mc cells) or RFP (expressed by RFP-PC-3/S cells) was visualized microscopically. Only GFP signal, but not RFP signal, was detected in lung tumors. (B) Only PC-3/Mc cells, but not PC-3/S cells, colonize adrenal glands after i.c. joint inoculation. Thirty-three days after inoculation, mice were sacrificed and adrenal metastatic tumors frozen and processed for fluorescent visualization of GFP or RFP and for immunofluorescent detection of firefly luciferase (as a marker common to both cell types). Samples were counterstained for nuclei with DAPI. Only GFP signal, but not RFP signal, was detected in adrenal metastases. Scale bars: 50 μm.