Figure 5. Constitutive overexpression of Snai1 inhibits local growth, metastatic spread, and distant organ colonization of PC-3/Mc cells.

(A) Overexpression of Snai1 strongly inhibited local growth and metastatic spread after orthotopic prostatic implantation of PC-3/Mc-SNAI1 cells (1.0 × 10⁵) in 6-week-old male NOD-SCID mice. Anterior or posterior halves were imaged independently for enhanced resolution. Middle panel: growth curves of orthotopic tumors, with photon counts normalized to values on day 0. Right panel: Kaplan-Meier plots for metastasis-free mice. (B) Overexpression of Snai1 strongly inhibited the growth of PC-3/Mc cells (2.5 × 10⁵) grafted i.m. Mice grafted with control PC-3/Mc cells were euthanized at day 22 after grafting. Bottom panel: growth curve at the i.m. implantation site. (C) Overexpression of Snai1 prevented lung colonization of PC-3/Mc cells (2.5 × 10⁵) inoculated i.v. Bottom panel: Kaplan-Meier plots for lung colonization–free mice. (D) Overexpression of Snai1 suppressed bone colonization of PC-3/Mc cells (2.0 × 10⁵) inoculated i.c. Bottom panel: Kaplan-Meier plots for bone colonization–free mice. Results are expressed as mean ± SEM.