(A) Downregulation of E-cadherin and upregulation of fibronectin
in PC-3/Mc cells after implantation in NOD-SCID mice. Seven days after i.m.
implantation, PC-3/Mc cells, homogeneously positive for E-cadherin and
negative for fibronectin in culture prior to implantation, become
heterogeneous for expression of membrane-associated E-cadherin, as
determined by immunohistochemistry (left panel), and downregulate E-cadherin
and upregulate fibronectin, as determined by Western blotting (right panel).
Lanes separated by the white lines were run on the same gel but were
noncontiguous. Scale bar: 100 μm. (B) PC-3/Mc cells
that had metastasized to lungs after i.v. injection were largely positive
for nuclear SOX2 and membrane-associated E-cadherin, as determined by
immunohistochemistry. Scale bars: 100 μm.