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. 2012 Apr 16;122(5):1849–1868. doi: 10.1172/JCI59218

Figure 9. Downregulation of E-cadherin from PC-3/Mc cells at primary implantation sites and maintenance of its expression in lung metastasis.

Figure 9

(A) Downregulation of E-cadherin and upregulation of fibronectin in PC-3/Mc cells after implantation in NOD-SCID mice. Seven days after i.m. implantation, PC-3/Mc cells, homogeneously positive for E-cadherin and negative for fibronectin in culture prior to implantation, become heterogeneous for expression of membrane-associated E-cadherin, as determined by immunohistochemistry (left panel), and downregulate E-cadherin and upregulate fibronectin, as determined by Western blotting (right panel). Lanes separated by the white lines were run on the same gel but were noncontiguous. Scale bar: 100 μm. (B) PC-3/Mc cells that had metastasized to lungs after i.v. injection were largely positive for nuclear SOX2 and membrane-associated E-cadherin, as determined by immunohistochemistry. Scale bars: 100 μm.