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. 2012 Mar 29;287(21):17318–17330. doi: 10.1074/jbc.M112.340885

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — The anti-phagocytic effect of extracted serum glycoprotein-rich fraction. Human monocyte-derived macrophages (a) or fresh monocytes (b, c, and d) were pretreated with 0.5 mm ATP for 15 min, 20 μm CytD for 30 min, or ∼250 μg/ml glycoprotein-rich serum fraction for 1 min (a concentration at which the phagocytosis of non-opsonized YG beads by monocytes was completely inhibited). a, phagocytosis of CFSE-labeled apoptotic lymphocytes by autologous monocyte-derived macrophages is shown. b, phagocytosis of CFSE-labeled live S. aureus by human monocytes is shown. c and d, phagocytosis of heat-killed Alexa 488-conjugated S. aureus (c) or E. coli. (d) by human monocytes is shown.