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. 2012 Apr 9;287(21):17860–17869. doi: 10.1074/jbc.M112.347716

FIGURE 1.

FIGURE 1.

Involvement of Gli1 in Hh-mediated osteoblast differentiation. A, ALP staining and Alp mRNA expression determined by real-time RT-PCR in perichondrial cells from WT, Gli1−/−, Gli2−/−, and Gli3−/− mouse embryos (E17.5). Cells were obtained form three pairs of WT and mutant mice. Cells were treated with SAG for 7 days for ALP staining and for 3 days for real-time RT-PCR analysis. *, p < 0.05. B, mRNA expression of osteoblast marker genes determined by real-time RT-PCR in C3H10T1/2 cells. Cells were transfected with the GFP (1 μg) or GLI1 (0.1 or 1 μg) plasmid and cultured for 48 h. C, mRNA expression of osteoblast marker genes determined by real-time RT-PCR in C3H10T1/2 cells. After being transfected with shRNA for GFP or Gli1, the cells were cultured for 12 h, followed by exposure to SAG (1 μm) for 48 h. shGli1, Gli1 shRNA. *, p < 0.05. For A–C, data are means ± S.D. of triplicate wells, and representative data of independent experiments are shown.