FIGURE 4.

Effects of Hh-Gli1 signaling on expression of osteoblast marker genes in WT and Runx2^−/− perichondrial cells. mRNA expression of osteoblast marker genes and Runx2 was determined by real-time RT-PCR analysis in perichondrial cells from WT or Runx2^−/− mouse embryos (E17.5). A, cells were treated with SAG (1 μm) for 6 days. B, cells were transfected with the GFP (1 μg) or GLI1 (1 μg) plasmid and cultured for 48 h. N.D., none detected. Data are means ± S.D. of triplicate wells, and representative data of independent experiments are shown.