FIGURE 1.

TNFα stimulates cytotoxic ROS formation in Jurkat cells. A, TNFα rapidly stimulates ROS formation in cultured Jurkat lymphoid cells. Amplex Red® fluorescence at 590 nm in the absence of or after the addition of 40 ng/ml TNFα with or without DPI addition. n = 3. B, DPI suppresses TNFα cytotoxicity. Jurkat cells were treated with TNFα for 24 h or not after a 30-min prior exposure to 20 μm DPI. Cell death was assessed as described under “Materials and Methods.” n = 3; *, p < 0.05. Error bars, S.E.