Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 20;287(21):17693–17705. doi: 10.1074/jbc.M111.300012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — Phospholipid hydroperoxides are required precursors for TNFα-stimulated Az-PC accumulation. A, DPI abolishes TNFα-induced Az-PC accumulation. Jurkat cells were treated as stated with DPI and TNFα or not for 24 h before Az-PC was determined by mass spectrometry as in Fig. 6. n = 3; *, p < 0.05. B, NOx4 knockdown suppressed TNFα-initiated phospholipid fragmentation. Az-PC was quantified by mass spectrometry in cells with reduced NOx4 or cells expressing scrambled siRNA. n = 6; *, p < 0.05. C, siRNA knockdown of GPx4 enhances TNFα-initiated phospholipid truncation. GPx4 RNA and protein were reduced by siRNA before Az-PC was quantified. n = 6; *, p < 0.05. D, GPx4 overexpression abolishes TNFα-induced accumulation of Az-PC. Az-PC content after transient GPx4 or empty vector transfection with or without TNFα stimulation. n = 6; *, p < 0.05. Error bars, S.E.