Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 30;287(21):17120–17129. doi: 10.1074/jbc.M111.311902

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — ARF and L11 form a complex with MDM2 and p53. A, H1299 cells were transfected with various combinations of plasmids encoding Flag-L11, V5-ARF, HA-MDM2, and p53 as indicated. Cell lysates were immunoprecipitated with anti-Flag or anti-V5 antibody, followed by IB with antibodies as indicated. B, sequential co-IP of L11 and ARF with MDM2 and p53. H1299 cells transfected with Flag-L11, V5-ARF, HA-MDM2, and p53 plasmids were subjected to IPs with control mouse IgG (lane 2) or anti-Flag (M2) antibody (lane 3), followed by elution with Flag peptide. 10% of the elution was loaded (lanes 2 and 3, respectively). The remaining 90% of the anti-Flag elution was immunoprecipitated with anti-V5 antibody (lane 5) or control IgG (lane 4), followed by IB with the indicated antibodies.