FIGURE 6.
Overexpression of Mcl-1 or Bcl-xL attenuates p14ARF-induced apoptosis. A, an pRTS1-Mcl-1 vector was stably introduced into DU145 cells to allow for the conditional expression of Mcl-1 in the presence of tetracycline (doxycycline, Dox). Cells were infected with indicated adenoviral vectors at 50 m.o.i. or mock-treated (Co). B, DU145 cells stably overexpressing Bcl-xL were infected with Ad-ARF or Ad-lacZ (both 50 m.o.i.) or mock-treated in parallel with empty vector-transduced controls (mock). Apoptosis was measured by flow cytometric detection of fragmented nuclear DNA at 72 h. The relative number of apoptotic cells is given. Error bars represent mean ± S.D. from triplicates.