FIGURE 1.

Tetracycline-dependent growth of the conditional mutant crd1Δpsd1Δ. A, 10-fold serial dilutions of cell suspensions were spotted on YPD plates supplemented with 200 μg/ml tetracycline (Tet) where indicated and incubated at 30° C. B, Cells were grown in YPD for 12 h in the presence or absence of tetracycline. Steady state labeling, phospholipid extraction, one-dimensional TLC, phosphorimaging, and quantification were carried out as described under “Experimental Procedures.” CL levels are quantified as percent of total phospholipids. Mean values ± S.D. of two independent experiments are shown. PC, phosphatidylcholine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; PA, phosphatidic acid.