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. 2012 Mar 21;287(21):17628–17636. doi: 10.1074/jbc.M112.340059

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Analysis of purified TK protein constructs on a 12% SDS polyacrylamide gel. The lanes contain molecular mass marker, purified His₆-tagged T. brucei TK (TbTK lane), domain 1 before and after removal of the MBP fusion partner (D1 lanes), domain 2 before and after removal of the TrxA fusion partner (D2 lanes), and human TK1 before and after removal of the Z protein fusion partner (hTK1 lanes). Theoretical molecular masses are 52 kDa (TbTK), 63 kDa (MBP-D1), 23 kDa (D1), 42 kDa (TrxA-D2), 28.5 kDa (D2), 35 kDa (Z-hTK1), and 25 kDa (hTK1). The molecular mass marker used is the Pageruler^TM prestained protein ladder (catalog no. SM0671, Fermentas Life Science).