FIGURE 2.

Proton ¹H-NMR spectra of metabolic end products excreted by procyclic cell lines incubated with d-glucose. The parental EATRO1125.T7T, Δach, tetracycline-induced (.i), or non-induced (.ni) ^RNAiASCT and Δach/^RNAiASCT cell lines were incubated with 4 mm d-glucose in PBS/NaHCO₃ buffer for 6 h. Each spectrum corresponds to one representative experiment from a set of at least three. The spectrum detail ranging from 1.5 to 3 ppm is shown. The resonances indicated are acetate (A), pyruvate (P), and succinate (S).