FIGURE 6.

The interaction modeling of the TRPC4 C terminus (amino acids 701–720) with Gα_i2. A, a model of association between Gα_i2 and TRPC4. An ionic interaction was assumed between the two proteins. Amino acids from 671 to 758 of TRPC4 are shown. B, summary of effects of Gα_i2^Q205L on current by mTRPC4 and mTRPC4 mutants in the presence and absence of GTPγS stimulation. mTRPC4 mutants were substituted at CIRB residues (R711K, R711A, N712R, K715A, and R716A). All current densities represent maximal current peaks (subtracted Cs⁺ basal current) at −60 mV in Cs⁺ solution and are indicated by means ± S.E. Statistical significance was denoted by an asterisk (p < 0.05).