FIGURE 1.
TtuB forms protein conjugates in T. thermophilus. A, working model for the dual roles of TtuB. TtuB was shown to function as a sulfur donor in s2T synthesis. The protein-modifier function of TtuB was examined in this study. B, left panel, anti-TtuB immunoblotting of extracts (22 μg) prepared from T. thermophilus cells cultured at 70οC in rich medium for the times indicated. Cells reached full growth at ∼13 h. The growth curve is shown in supplemental Fig. S2. Recombinant TtuB (rB) was loaded in lane 5. The Ponceau-stained membrane shows equal amounts of protein loaded (right panel).