TABLE 1.

Quantification of intracellular EGFP-CLASP2-(497–794) microtubule plus end association relative to the level of the wild-type construct

Mutations of SXIP motifs or phosphorylation sites are indicated in bold.

CLASP construct	Relative plus end association (mean ± S.D.; n = 10 cells)
SKIP-S-S-S—SRIP-S-S-S-S-S	1.00 ± 0.24
SKNN-S-S-S—SRIP-S-S-S-S-S	0.68 ± 0.11
SKIP-S-S-S—SRNN-S-S-S-S-S	0.44 ± 0.16
SKNN-S-S-S—SRNN-S-S-S-S-S	No plus-end-tracking
SKIP-A-A-A—SRIP-A-A-A-A-A	2.73 ± 0.54
SKNN-A-A-A—SRIP-A-A-A-A-A	1.74 ± 0.26
SKIP-A-A-A—SRNN-A-A-A-A-A	1.68 ± 0.40
SKNN-A-A-A—SRNN-A-A-A-A-A	No plus-end-tracking
SKNN-A-A-A—SRIP-S-S-S-S-S	1.75 ± 0.39
SKNN-S-S-S—SRIP-A-A-A-A-A	1.93 ± 0.29
SKIP-A-A-A—SRNN-S-S-S-S-S	1.70 ± 0.33
SKIP-S-S-S—SRNN-A-A-A-A-A	1.75 ± 0.43