Skip to main content
. 2012 Mar 30;287(21):17130–17139. doi: 10.1074/jbc.M111.338855

FIGURE 2.

FIGURE 2.

Arsenic reduces abundance and function of CFTR in airway epithelial cells. A, arsenic reduces the abundance of CFTR in the apical membrane and cell lysate. CFBE cells were treated with varying doses of arsenic (0.1–50 ppb) or vehicle (water) for 4 h, and cell surface biotinylation and Western blot analyses were performed. Representative blots are shown in the upper panels, and the summary of the data is shown in the lower panel. AP CFTR is apical membrane CFTR (black bars). WCL CFTR is whole cell lysate CFTR (striped bars). Data are normalized for ezrin in whole cell lysates and presented as the percentage of control (C). n = 4/group. *, p < 0.05 versus control. B, arsenic reduces the CFTR-mediated short circuit current. Polarized CFBE cells were treated with arsenic (10 ppb of arsenic for 4 h before Ussing chamber studies). Data are reported as the short circuit current inhibited by the CFTR channel blocker CFTR-172 in forskolin (10 μm)-treated cells (ΔIsc). n = 4/group. *, p < 0.05.