FIGURE 5.

Arsenic does not alter USP10 DUB activity. Cells were treated with arsenic (2 ppb) or vehicle (water) for 4 h, cells were lysed, and the lysates were incubated with the HA-UbVME probe to identify active DUBs. The HA-UbVME-DUB complex was immunoprecipitated with an anti-HA antibody, and the immunoprecipitated complex was analyzed by Western blot analysis using an anti-USP10 antibody. The nonimmune IgG did not immunoprecipitate USP10 and thus served as a negative control (data not shown). n = 3/group. Representative blots are shown above quantitation.