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. 2012 Mar 27;287(21):17016–17028. doi: 10.1074/jbc.M112.350470

FIGURE 5.

FIGURE 5.

NDRG1 knock-down mimics the TGF-β-induced EMT. A, NDRG1 knock-down HT29 cells (clone 1), DU145 cells (clone 5), and their respective control cells transfected with scrambled control shRNA were treated with or without TGF-β (5 ng/ml) for 96 h and 48 h, respectively. The efficacy of NDRG1 knock-down and the expression of EMT markers (i.e. E-cadherin, β-catenin, and vimentin) were analyzed by Western blotting. B, bright field microscopy and immunofluorescence showed that NDRG1 knock-down mimics the EMT phenotype in both HT29 and DU145 cells. Scale bars: 100 μm for bright field and 20 μm for immunofluorescence. C and D, cell migration and invasion assays were performed as described in Fig. 2, D and E. Scale bars: 200 μm. Data were repeated 3–5 times, and the values in histograms represent mean ± S.D. (3–5 experiments). *, relative to vector control without TGF-β; #, relative to vector control with TGF-β. **, p < 0.01; ***, p < 0.001; ##, p < 0.01; ###, p < 0.001.