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Effect of GLD on GAP activity of AGAP1. A, His10AGAP1 was titrated into a reaction containing 0.4 μm myrArf1·GTP, 500 μm large unilamellar vesicles, and either 1 mm GTP or 1 mm GDP. B, His10[ΔGLD]AGAP1 was titrated into the reaction as described in A.
