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Effect of nucleotides on GAP activity of AGAP1
His10AGAP1 was titrated into a reaction containing 0.4 μm myrArf1·GTP, 500 μm LUVs, and 1 mm concentrations of the indicated nucleotide. The results are the average and S.E. of three experiments. These experiments were independent of those presented in Fig. 1.
| Protein | C50 |
|||
|---|---|---|---|---|
| GTP | GDP | ATP | ADP | |
| nm | ||||
| His10AGAP1 | 2.0 ± 1.1 | 2.0 ± 1.3 | 3.1 ± 1.4 | 2.0 ± 1.0 |
| [ΔGLD]AGAP1 | 2.1 ± 1.2 | 1.8 ± 1.3 | ||
