TABLE 3.

Comparison of RhoA and Rac1 as activators of AGAP1

RhoA and Rac1 were titrated into a reaction mixture contain 2.2 nm His₁₀AGAP1 and 0.2 μm [α−³²P]GTP·myrArf1. A plot of GAP activity vs. RhoA or Rac1 was fit to a hyperbola to determine the amount required to achieve one-half maximal effect (EC₅₀). The results are the mean ± S.E. for three experiments. The means were compared by Student's t test.

EC50
RhoA	Rac1
nm
91 ± 24	197 ± 36a

^a, p < 0.05.