TABLE 4.

Effect of RhoA on the Arf specificity of AGAP1

AGAP1 was titrated into a GAP reaction containing either 0.2 μm myrArf1· [α³²P]GTP or myrArf6· [α³²P]GTP and 0.5 μm RhoA·GDP as indicated. The concentration of AGAP1 required to achieve 50% hydrolysis was determined from curves. The means for three experiments are presented. The results were analyzed by Student's t test.

Protein	C50
	AGAP1	AGAP1+RhoA
	nm
Arf1	4.2 ± 0.9	0.32 ± 0.04a
Arf6	3.6 ± 0.7	23 ± 13b

^a, p < 0.001 compared to no RhoA.

^b, p < 0.05 compared to no RhoA.